Frequently Asked Questions

The simulator uses mechanistic models based on physical first-principles equations to simulate chromatography experiments. You define your system using a drag-and-drop flowsheet editor, specify your column, resin, and operating conditions, and the simulator solves the underlying transport and adsorption equations to predict chromatogram outputs. Learn more about mechanistic modeling.

All types of biomolecules are supported, including:

• Monoclonal antibodies (mAbs)
• Adeno-associated viruses (AAVs)
• Nanobodies
• Fusion proteins
• Plasmids
• Oligonucleotides
• And more

Both bind-elute and flow-through operation modes are supported, including:

• Ion exchange (IEX)
• Hydrophobic interaction (HIC)
• Affinity
• Size exclusion (SEC)
• Mixed-mode (MMC)

Each interaction method is described by a specific binding model. Learn more about binding models.

No. Efflux is a fully web-based platform that runs in your browser. There is no software to install or maintain, and you can access your simulations from any device.

Yes. One of the key advantages of mechanistic modeling is the ability to predict chromatography behavior at different scales. You can develop and calibrate your model at lab scale and use it to predict performance at pilot or manufacturing scale.

Yes. Efflux supports team collaboration, allowing multiple members within your organization to access and work on shared projects.

Efflux supports importing experimental data from common formats including CSV, Excel, and UNICORN files directly. All simulation results can be exported for further analysis.

Mechanistic modeling uses physics-based equations to simulate chromatography experiments. Physical mechanisms such as convection, diffusion, mass transfer, and adsorption are described using first-principles equations. By using mechanistic modeling, the number of real-world experiments can be reduced compared to statistical modeling approaches, while greatly improving process understanding. Learn more about mechanistic modeling.

Mechanistic modeling is based on physical equations that describe the underlying mechanisms within the chromatography column. Statistical models (like DoE) are based on correlations between inputs and outputs and cannot explain the underlying process mechanisms. The advantage of mechanistic models is that they typically require fewer experiments to optimize process parameters, while simultaneously providing a better process understanding. Read about the science behind mechanistic modeling.

The simulations are based on mechanistic models that describe the underlying physical processes within the column. When calibrated with a small number of experiments, the models can accurately predict chromatography behavior across a wide range of operating conditions, including conditions not tested experimentally.

The number of calibration experiments depends on the complexity of your process. Typically, 5-8 well-designed experiments are sufficient for model calibration. We work with you to design an efficient experimental plan that minimizes resource usage while ensuring model quality. Learn about the types of experiments used for modeling.

Efflux offers flexible pricing plans tailored to individual teams and organizations.

Yes. You can try Efflux and explore the platform before committing to a paid plan. Please contact us to get started.

Yes. We offer special pricing for academic institutions and non-profit organizations. Please contact us to discuss your specific needs.

Contract modeling project costs depend on the scope and complexity of the work. We provide a detailed cost estimate during the initial consultation. Learn more about contract modeling.

You can request access to Efflux directly from our website. Once approved, you will receive an email with instructions to set up your account and start simulating.

All you need is a web browser and experimental data from your chromatography process. Efflux runs entirely in the cloud, so there is no software to install. Read our getting started guide.

We can help you with virtually any downstream processing projects. However, our expertise is within process development, process intensification, and tech-transfer.

Project timelines vary based on complexity and scope, but typically, a project takes 4-8 weeks from initial meeting to project completion. We provide a timeline estimate during the initial consultation.

Yes, we can provide documentation and reports suitable for use in regulatory submissions. Our models and optimization studies can support process characterization, design space definition, and control strategy development in line with ICH Q8-Q12 guidelines.

Yes. We take data security and confidentiality very seriously. All project data is protected under a strict confidentiality agreement, and we use secure systems for data transfer and storage. Learn more about our security practices.

You will receive a calibrated mechanistic model of your chromatography process, along with a detailed report documenting the model parameters, validation results, and any optimization recommendations. We can tailor deliverables to your specific needs.

We offer support via email and video calls. Our team is available to help you with technical questions, troubleshooting, and best practices for chromatography modeling. Get in touch with us.

Yes. We provide onboarding sessions to help you get started with the platform. We also offer training workshops on mechanistic modeling fundamentals and advanced techniques tailored to your team's needs.

Yes. We have an extensive library of articles covering everything from getting started to advanced topics like binding models and process optimization. Browse our learning resources.

We aim to respond to all support inquiries within one business day. For urgent issues, we prioritize faster turnaround. Contact us.